Triphosphates like CTP and ATP are high-energy molecules; but ATP is involved in many more metabolic reactions than CTP. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of CTP synthetase. These variations probably reflect the species differences in cytidine deaminase, the enzyme that converts cytidine to uridine in the body. It is used in the synthesis of RNA by RNA polymerases. The enzyme Cytidine 5’-triphosphate (CTP) synthetase (EC 6.3.4.2) catalyzes a critical reaction in pyrimidine nucleotide biosynthesis as well as phospholipid formation [1-3]. It is used in the synthesis of RNA by RNA polymerases. Generally, these enzymes hydrolyze some nucleoside 3',5'-cyclic phosphate to some nucleoside 5'-phosphate thus controlling the cellular levels of the cyclic second messengers and the rates of their degradation. Cytidine triphosphate synthetase (CTPS) catalyzes the rate-limiting step in the de novo synthesis of CTP, and both the yeast and human enzymes have been reported to be regulated by protein kinase A or protein kinase C phosphorylation. Nucleotide structure A nucleotide is comprised of a ring of nitrogen, carbon and oxygen atoms, a five carbon sugar (together referred to as a nucleoside) and one or more phosphate groups. Academic disciplines Business Concepts Crime Culture Economy Education Energy Events Food and drink Geography … HU also exerts a synergistic effect with nucleoside analogs used in the treatment of human immunodeficiency virus (HIV)-1. In addition, DP-55 has a requirement for uracil (uridine). Cytidine triphosphate is a pyrimidine nucleoside triphosphate. 2',2'-Difluorodeoxycytidine (dFdC, Gemcitabine) is a deoxycytidine analogue which, after phosphorylation to the 5'-di- and 5'-triphosphate (dFdCTP), induces inhibition of DNA synthesis and cell death. Cytidine 5′-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is analogous to the better-known ATP, but with cytidine as the base instead of adenine. The enzymatic activity could not be dissociated from platelet particles and the greatest specific activity was found in the membrane fraction. Thenucleoside triphosphate pools oftwocytidine auxotrophic mutants ofSal-monella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. novel compounds cytidine diphosphate choline and cytidine diphosphate ethanolamine are examples, has been found to play an essential r&e in the biosynthesis of phospholipides. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. CTP synthetase is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Here we report the biochemical characterization of Trypanosoma brucei CDA as an enzyme within the tetrameric class of the CDA family that efficiently deaminates cytidine, deoxycytidine, and the nucleoside analogue 5-methyl-2′-deoxycytidine. Sigma-Aldrich (13) Supelco (1) Manufacturer Name. The T7 RNA polymerase synthesis of modified RNA can be very efficiently used for synthesis of modified RNA but the method has constraints in the sequence of the first three nucleotides of the transcript, which must contain a non-modified G in the +1 position. The major difference between the two molecules is the base used, which in CTP is cytosine. LOpezG.-Coviella I. and Wurtman R. J. Enhancement by cytidine of membrane phospholipid synthesis. CDP indicates cytidine diphosphate; CMP, cytidine monophosphate; and UTP, uridine triphosphate. Size Price Stock Quantity ; 25mg: EUR 95 In stock: Bulk Size: Bulk Discount: Bulk Inquiry. Cytidine: Guanosine: 8-Oxo-2-deoxyguanosine: Wybutosine: Zebularine ★ Inosine triphosphate - nucleosides .. Add an external link to your content for free. Reaction mechanism. CTP is a substrate in the synthesis of RNA. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Convenient Syntheses of Cytidine 5'-Triphosphate (CTP), Guanosine 5'-Triphosphate (GTP), and Uridine 5'-Triphosphate (UTP) and Their Use in the Preparation of UDP-Glucose, UDP-Glucuronic Acid, and GDP-Mannose An inhibitor of cytidine triphosphate synthesis in human colon carcinoma cells. Tetrahedron 2003 , 59 (37) , 7315-7322. 36051-68-0 Cytidine 5'-triphosphate is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. CTP acts as an inhibitor of the enzyme aspartate carbamoyltransferase, which is used in pyrimidine biosynthesis. Endothelial cells (ECs) have a salvage pathway to synthesize CTP using cytidine, which preserves or promotes proliferation of ECs. Exogenous cytidine, acting via cytidine triphosphate, can thus affect the synthesis and levels of cell membrane phospholipids. The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. Convenient Syntheses of Cytidine 5'-Triphosphate, Guanosine 5'-Triphosphate, and Uridine S'-Triphosphate and Their Use in the Preparation of UDP-glucose, UDP-glucuronic Acid, and GDP-mannose Ethan S. Simon,l Sven Grabowski,2 and George M. Whitesides* Department of Chemistry, Haruard Uniuersity, Cambridge, Massachusetts 02138 Receiued September 20, 1989 This paper compares … Cytidine triphosphate synthetase (CTPS) is the rate-limiting enzyme in de novo CTP synthesis and is required for the formation of RNA, DNA, and phospholipids. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Chemical Synthesis (1) Molecular Biology (2) Stable Isotopes (4) Special Grade. Synthesis of 8-vinyladenosine 5′-di- and 5′-triphosphate: evaluation of the diphosphate compound on ribonucleotide reductase. Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. The Km for cytidine triphosphate was 0.16 mmole/liter and the apparent Km for phosphatidic acid was 6.2 mmoles/liter. certified reference material (1) pharmaceutical primary standard (6) pharmaceutical secondary standard (1) Brand. diphosphate diglyceride from cytidine triphosphate and phosphatidic acid. 3'5'-cyclic nucleotide phosphodiesterases are a family of phosphodiesterases. 99% (1) 98% (2) 96% (1) 95% (6) 90 - 94% (1) Physical Form. A subsidiary of Cambridge Isotope Laboratories, Inc. Informations; About Us; Contact Us; Distributors; Custom Synthesis… ; Voûte, P.A.T. This paper will describe the enzymatic synthesis of cytidine diphosphate choline and cytidine diphosphate ethanol- Interactive image; Interactive … Bothmutants,DP45andDP-55,aredefectivein cytidinedeaminaseand cytidine triphosphate (CTP) synthase. 4NH3: MW: 554,26: Request For Quote. QTY : Add to my cart. Upon exposure of cells to 10(-6)M cCyd, cell viability was reduced to 20% of control, whereas cytocidal activity was not present after 2 hr … CTP is a substrate in the synthesis of RNA. Cytidine triphosphate (CTP) synthetase is a key enzyme for the synthesis of cytosine (deoxy)ribonucleotides, catalysing the conversion of uridine triphosphate (UTP) into CTP, and has a high activity in several malignancies. Cytidine triphosphate Names IUPAC name [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl(hydroxy-phosphonooxyphosphoryl)hydrogen phosphate. Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine. It is used in the synthesis of RNA by RNA polymerases. Key Words: Acetylcholine-Choline-Cytidine-PC 12 cells-Phosphatidylcholine-Phospholipids. Glazer RI, Knode MC, Lim MI, Marquez VE. Catalog No.S6077 CAS No. Now, however, CTP may be useful in the development of … It is a precursor of cytidine triphosphate (CTP) needed in the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) biosynthetic pathways. Other names CTP; Cytidine-5'-triphosphate; Cytidine 5'-(tetrahydrogen triphosphate) Identifiers CAS Number. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Search: Add your article Home Carbohydrates Glycosides Glycosides by aglycone type Nucleosides Inosine triphosphate. ; Vreken, P.; Kuilenburg, A.B.P. Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. CTP is a high-energy molecule similar to ATP, but its role as an energy coupler is limited to a much smaller subset of metabolic reactions. It is a substrate in the biosynthesis of RNA. Cytidine 5′-triphosphate (disodium salt) For research use only. 65-47-4 3D model . It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways.. Van 1999-01-04 00:00:00 Introduction CTP synthase (EC 6.3.4.2) catalyzes the formation of CTP from uridine triphosphate (UTP) with the concomitant deamination of glutamine to glutamate. BP (2) Cytiva (2) EDQM (3) Roche (1) USP (1) Purity. Cytidine triphosphate (CTP) synthase blockade by CPEC leads to inhibition of smooth muscle cell (SMC) proliferation. The mechanism of action of the cyclopentenyl analogue of cytidine, cCyd, was investigated in human colon carcinoma cell line HT-29. CTP (cytidine triphosphate) synthetase catalyzes the last committed step in pyrimidine nucleotide biosynthesis: ATP + UTP + glutamine → ADP + P i + CTP + glutamate . Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells Verschuur, A.C.; Gennip, A.H. Van; Muller, E.J. 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